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From: Leandro Bugnon <l.bugnon@gmail.com>
Date: Fri, 24 Jun 2011 22:34:51 -0300
Message-ID: <BANLkTikEF00e5_MJCuLkbR1=a=16zmVnKA@mail.gmail.com>
To: Bob Friesenhahn <bfriesen@simple.dallas.tx.us>
Cc: TIFF <tiff@lists.maptools.org>
Subject: Re: [Tiff] 16-bit image
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 thought that saving in TIFF file format would ensure that all values I
save, they'll show in the exact same way.

The only solution that I can think about is make a calibration image as the
first one, defining the min and max values. This isn't very atractive, but I
have to use ImageJ becouse there we can develope all the processing task.

Maybe I should try some variation, like OME-TIFF, becouse some parameters
cant be saved with tags, like the Z Resolution.

Thank you again,

2011/6/24 Bob Friesenhahn <bfriesen@simple.dallas.tx.us>

> On Fri, 24 Jun 2011, Leandro Bugnon wrote:
>
>  The image was made that way intentionally. My problem is that if I have a
>> stack with 2 images or slices, some of
>> the viewers I've tryed define the display range between the min and max of
>> the first image. In the test image,
>>  the value 500 is represented in black  and 700 in white, so the second
>> image (1000-1400) it's completely white.
>> I cannot scale the image becouse it's for cuantitative microscopy, and the
>> values goes from 0 to 2^14, so if I
>> have a image from 500-700, it have to be almost dark and darker than the
>> second image. I supposed that had some
>> tag missed, but it seems not. The pixel values are correctly saved, the
>> problems seems the visualization info to
>> show them in a scale from 0 to 2^16 ( I can scale from 0-2^14 to 0-2^16
>> but I cannot scale anything to 0-2^16
>> becouse I'm distorting the information).
>>
>
> The traditional sort of image viewers you tried (imageJ and irfanview) are
> really intended for images scaled to ranges for normal viewing on an 8-bit
> display.  You will need a scientific/remote-sensing sort of display
> application to make sense of your images.
>
> Bob
> --
> Bob Friesenhahn
>
> bfriesen@simple.dallas.tx.us, http://www.simplesystems.org/**
> users/bfriesen/ <http://www.simplesystems.org/users/bfriesen/>
> GraphicsMagick Maintainer,    http://www.GraphicsMagick.org/
>



-- 

Leandro

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=A0thought that saving in TIFF file format would ensure that all values I s=
ave, they&#39;ll show in the exact same way.=A0<div><br></div><div>The only=
 solution that I can think about is make a calibration image as the first o=
ne, defining the min and max values. This isn&#39;t very atractive, but I h=
ave to use ImageJ becouse there we can develope all the processing task.=A0=
</div>

<div><br></div><div>Maybe I should try some variation, like OME-TIFF, becou=
se some parameters cant be saved with tags, like the Z Resolution.</div><di=
v><br></div><div>Thank you again,</div><br><div class=3D"gmail_quote">2011/=
6/24 Bob Friesenhahn <span dir=3D"ltr">&lt;<a href=3D"mailto:bfriesen@simpl=
e.dallas.tx.us">bfriesen@simple.dallas.tx.us</a>&gt;</span><br>

<blockquote class=3D"gmail_quote" style=3D"margin:0 0 0 .8ex;border-left:1p=
x #ccc solid;padding-left:1ex;"><div class=3D"im">On Fri, 24 Jun 2011, Lean=
dro Bugnon wrote:<br>
<br>
</div><div class=3D"im"><blockquote class=3D"gmail_quote" style=3D"margin:0=
 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
The image was made that way intentionally. My problem is that if I have a s=
tack with 2 images or slices, some of<br>
the viewers I&#39;ve tryed define the display range between the min and max=
 of the first image. In the test image,<br>
=A0the value 500 is represented in black =A0and 700 in white, so the second=
 image (1000-1400) it&#39;s completely white.=A0<br>
I cannot scale the image becouse it&#39;s for cuantitative microscopy, and =
the values goes from 0 to 2^14, so if I<br>
have a image from 500-700, it have to be almost dark and darker than the se=
cond image. I supposed that had some<br>
tag missed, but it seems not. The pixel values are correctly saved, the pro=
blems seems the visualization info to<br>
show them in a scale from 0 to 2^16 ( I can scale from 0-2^14 to 0-2^16 but=
 I cannot scale anything to 0-2^16<br>
becouse I&#39;m distorting the information).<br>
</blockquote>
<br></div>
The traditional sort of image viewers you tried (imageJ and irfanview) are =
really intended for images scaled to ranges for normal viewing on an 8-bit =
display. =A0You will need a scientific/remote-sensing sort of display appli=
cation to make sense of your images.<br>


<br>
Bob<br>
-- <br><font color=3D"#888888">
Bob Friesenhahn</font><div><div></div><div class=3D"h5"><br>
<a href=3D"mailto:bfriesen@simple.dallas.tx.us" target=3D"_blank">bfriesen@=
simple.dallas.tx.us</a>, <a href=3D"http://www.simplesystems.org/users/bfri=
esen/" target=3D"_blank">http://www.simplesystems.org/<u></u>users/bfriesen=
/</a><br>


GraphicsMagick Maintainer, =A0 =A0<a href=3D"http://www.GraphicsMagick.org/=
" target=3D"_blank">http://www.GraphicsMagick.org/</a></div></div></blockqu=
ote></div><br><br clear=3D"all"><br>-- <br><br>Leandro <br>

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